Quantitative Forensic Analysis of Opiates,Opioids, a

内容节点: 概述; 实验/设备条件; 样品提取; 实验/操作方法; 实验结果/结论; 仪器/耗材清单

A forensic method for analysis of opiates, opioids, and their metabolites without hydrolysis has been developed using the Prelude SPLC system and TSQ Endura MS. By eliminating the hydrolysis step, the sample preparation time and analysis cost was drastically reduced. The LC method on the Prelude SPLC system/TSQ Endura MS provided ample resolution for all isobaric compounds andan outstanding increase in overall speed of analysis. The high sensitivity that the TSQ Endura MS provided allowed for low limits of quantitation of even the least responsive analytes, like the gluronidated metabolites. The fast SRM acquisition rate yielded a successful, simultaneous analysis of 19 compounds with 10 internal standards. 赛默飞TSQ Endura三重四极杆质谱仪赛默飞Prelude SPLC样品前处理及液相系统

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