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仪器网/ 行业应用/ 解决方案/ Thermosensitive-polymer-coated magnetic nanoparticle

Thermosensitive-polymer-coated magnetic nanoparticle

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Adsorption and desorption behavior of Bovine Serum Albumin (BSA) on surface-modified magnetic nanoparticles covered with thermosensitive
polymer (PNIPAM) was investigated as a function of temperature, pH, and ionic strength. Functionalization of surface-modified magnetic particles
was performed by seed polymerization using N-isopropylacrylamide (PNIPAM) as the main monomer. Characterization of these particles was
carried out using transmission electron micrography (TEM), and vibrating sample magnetometry (VSM). The adsorption results exhibited both
pH and temperature sensitivity. The results showed that the temperature effect on adsorption/desorption behavior was mainly dependent on the
properties of the particles surface. The effect of pH was also investigated and it was observed that a smaller amount of protein was adsorbed
at higher pH because of the electrostatic repulsive force between protein molecules and latex particles. The maximum amount of protein was
adsorbed near the isoelectric point of BSA. Desorption results showed that more protein was desorbed when adsorption was done at lower
temperatures and desorption efficiency was found to be higher than 80%.
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