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Pierce™ Nickel Coated Plates, White, 96-Well
- 品牌:美国赛默飞
- 货号:
- 产地:美国
- 供应商报价: 面议
- 赛默飞世尔科技实验室产品 更新时间:2024-06-20 15:37:53
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- 详细介绍
- 描述 Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.
Features of Pierce Nickel Coated Plates:
• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL
These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.
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Pierce™ Nickel Coated Plates, Black, 96-WellFor Research Use Only. Not for use in diagnostic procedures.图表Binding performance of nickel coated plates
Comparison of Thermo Scientific Pierce Nickel Coated Plates (Pierce) to Nickel-chelate plates from Brand Q in binding performance for His-tagged ATF fusion protein. Detection was accomplished with an HRP-conjugated anti-ATF antibody and TMB substrate." data-omni-action="View figure">Binding performance of nickel coated plates
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