Pierce™ Maleic Anhydride Activated Plates, Clear, 96-Well

描述 These Thermo Scientific Pierce Maleic Anhydride Activated 96-Well Plates are clear, 96-well plates that allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.

Features of Maleic Anhydride Activated Plates:

• Spontaneously react with primary amines
• A direct, simple approach to covalently attach molecules to the well surface of an EIA plate
• Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions

These plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (-NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours (Ref.1). Therefore, coating reactions are best performed at pH 8-9, and the reacted plates are best used at pH > 7 for ELISA and other methods.

Related Products
Pierce™ Maleic Anhydride Activated Plates, Clear, 8-Well Strip
Pierce™ Maleic Anhydride Activated Plates, White, 96-Well

For Research Use Only. Not for use in diagnostic procedures.

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