-
-
Pierce™ Protein Concentrator PES, 30K MWCO, 5-20 mL
- 品牌:美国赛默飞
- 货号:
- 产地:美国
- 供应商报价: 面议
- 赛默飞世尔科技实验室产品 更新时间:2024-06-20 15:37:53
-
企业性质生产商
入驻年限第6年
营业执照已审核
- 同类产品过滤器和过滤(46件)
联系方式:实验室产品和服务4008558699转8056
联系我们时请说明在仪器网(www.yiqi.com)上看到的!
-
为您推荐
- 详细介绍
- 描述 Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 30K and can be used for processing sample volumes ranging from 5 to 20 mL.
Features of Pierce Protein Concentrators PES:
• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning
For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.
Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.For Research Use Only. Not for use in diagnostic procedures.图表Comparison of flux curves for different MWCOs using 5-20 mL concentrators
Protein samples for each MWCO (3K, 10K, 30K, 100K) at approximately 0.25 mg/mL starting concentration were centrifuged in 5-20 mL Pierce Protein Concentrators PES at 5,000 x g for 90 min to determine the protein concentration rate. For 10K, 30K, and 100K MWCOs, >90% of the protein solution was concentrated within 15 min; for the 3K MWCO, this degree of concentration occurred within 90 min." data-omni-action="View figure">Comparison of flux curves for different MWCOs using 5-20 mL concentrators
Processing time and protein recovery using 5-20 mL concentrators
20 mL volumes of four different protein solutions were concentrated over 30-fold in a swinging bucket rotor at 5,000 x g at 25°C, using 3K, 10K, 30K, or 100K MWCO Pierce Protein Concentrators PES (5-20 mL)." data-omni-action="View figure">Processing time and protein recovery using 5-20 mL concentrators
- Pierce™ Protein Concentrator PES, 10K MWCO, 2-6 mL
- Pierce™ Protein Concentrator PES, 30K MWCO, 2-6 mL
- Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL
- Spectra Por Tube-A-Lyzer Dialysis Device, 100-500 MWCO
- Spectra Por Tube-A-Lyzer Dialysis Device, 3500-5000 MW
- Spectra Por Tube-A-Lyzer Dialysis Device, 8000-10000 M
- Spectra Por Tube-A-Lyzer Dialysis Device, 20000 MWCO,
- Vivaspin 2, 100 kDa MWCO PES
- Vivaspin 6, 50 kDa MWCO PES
- Vivaspin 500, 3 kDa MWCO PES
- Vivaspin 500, 50 kDa MWCO PES
- EPA Method 541 UCMR 4 Surrogate 1-butanol d10 1000 μg/