3.3. Microbial mapping The intensity of resonance Raman spectra of Cyt c allows for integration times below one second. Microbial mapping of an area of 50×50 μm2 with a resolution of 500 nm/pixel (10,000 single spectra) can be perform... 3.3. Microbial mapping
The intensity of resonance Raman spectra of Cyt c allows for
integration times below one second. Microbial mapping of an
area of 50×50 μm2 with a resolution of 500 nm/pixel (10,000
single spectra) can be performed in less than 3 h. A further
reduction of the measuring duration by a factor of 10 can be
achieved by utilisation of an EMCCD camera (Coates et al.,
2004).
Microbial mapping was done on granules from two different
sequencing batch reactors (SBR) (Gaul et al., 2006). The SBRs
are used to analyse control parameters for the anaerobic
ammonium oxidation. Fig. 7 shows two different microbial
colonies in the same granulum. A graphical analysis of the
spectroscopic data at 750 cm-1 (dominant resonant Raman
band of Cyt c) and 2900 cm-1 (CH stretching mode)
differentiated the two colonies. One colony shows stronger
Raman signals at 750 cm-1 (blue frame). Whereas the other
microorganisms have more amount of methyl or methylene
groups (yellow frame).
After the graphical analysis above, an average spectrum was
constructed for each colony. The resulting averaged spectra
were transferred to our Raman database of wastewater bacteria
created by OPUS. The software recognized the microorganisms
Fig. 5. Time series of N. eutropha Nm 57 which is captured by optical tweezers.
Time difference between spectra: 1 s; Laser power: 9 mW. The bleaching effect
due to photo-dissociation caused by laser radiation is easy to see.
Fig. 6. The spectral heterogeneity of hierarchical clusteringis strongly dependent to the integration time. Bacteria from the same strain are only groupedtogether if the
exposure time was the same.
246 R. P?tzold et al. / Journal of Microbiological Methods 72 (2008) 241–248
in the left colony as anammox bacteria, those in the upper right
corner as Nitrosomonas.
求一篇化工类英语文献,带中文翻译,谢谢,原文Z好是PDF格式 我这有文献 可是你要是要PDF的真没法上传
哪位高手能帮我简述固相肽合成技术,我对这方面一无所知啊,但是急用啊.谢谢啦 ziyirenjiajia
请问高人,Profibus怎么翻译?FoundationFieldbus怎么翻译?谢谢各位老师。...
具体问题是通电时有输出之后就停止工作,,如图,,谢谢了 只有逐个元件焊下来检测了,就会发现问题所在。
PLC不是一个软件类的他是一门技术,一门学科。 你看看视频之类就学好了那是完全不可能的。 做个Z简单的比方:
脉冲编码器:APC 增量脉冲编码器:SPC 两者一般都应用于速度控制或位置控制系统的检测元件. 旋转
我是一名电脑技术员,对主板测试卡的代码还不是很了解,那位大哥能给我介绍一下吗?还有你们使用测试卡的一些经验
详细看图 当液压控制口X或Y不通压力油时候,此阀相当于一个单向阀, 油液只能从A口流入顶开阀芯,然后
就是老师上课给留的题目哦~感激不尽 那个,一般上一届都有啊,我有,不过难得找了。你去问问学长。
首先检查一下饲料配方中各个原料的组成,其中的粗纤维不能太多,要含有油脂,有次粉等粘性原料。在保证原料有粘