pSecTag2 A&B&C说明书
- 上传人: 上海起发实验试剂有限公司 |大小:22KB|浏览:1542次|时间:2018-09-29
- 文档简介
- 您可能感兴趣的资料
-
上海炎熙生物为您提供支原体清除剂,帮您去除细胞培养中的支原体污染。采用双重杀菌机制,不易产生耐药。可同时去除
-
CCK-8试剂盒 WST-8 细胞增殖 细胞毒性,是一种基于WST-8的广泛应用于细胞增殖和细胞毒性的快速高
-
支原体PCR检测试剂盒 灵敏度高 使用方便 涵盖所有支原体种类,能在2-3小时内获得可靠的结果。特点:①特异
-
湿膜规使用说明书
-
说明书
-
Cobalt RapID空间位移拉曼-空间位移拉曼技术的原材料快速鉴别 (技术说明书)
-
电位滴定仪TIM8XX系列中英文对照说明书
-
上海田枫冷水机使用说明书
-
YHA-2310型便携式溶解氧测定仪说明书
-
YHXJ-2010型消解装置的使用说明书
pSecTag2A&B&C型号载体名称出品公司载体用途VPI0036pSecTag2AInvitrogen哺乳动物表达载体产品参数:MammalianSelection:ZeocinSequencingPrimer:T7FwdSequencingPrimerSequence:5'd[TAATACGACTCACTATAGGG]3'Tag:6XHis,myc载体抗性:氨苄青霉素(Ampicillin)载体描述:pSecTag2andpSecTag2/Hygroaremammalianexpressionvectorsdesignedforthesecretion,purification,anddetectionoffusionproteins.EachvectorhasalargemultiplecloningsiteinthreereadingframestosimplifycloninginframewiththeN-terminalsecretionsignal.Thevectors(Figure1)offerthefollowingfeatures:SecretionsignalfromtheV-J2-CregionofthemouseIgkappa-chainforefficientsecretionofrecombinantproteins(Figure2)Cytomegalovirus(CMV)promoterforhigh-levelconstitutiveexpressionC-terminalpolyhistidine(6xHis)tagforrapidpurificationwithnickel-chelatingresinanddetectionwithanAnti-His(C-term)AntibodyC-terminalc-mycepitopefordetectionwithanAnti-mycAntibodyBovineGrowthHormone(BGH)polyadenylationsignalandtranscriptionterminationsequencetoenhancemRNAstabilitySV40originforepisomalreplicationandsimplevectorrescueincelllinesexpressingthelargeTantigen(e.g.,COS-1,COS-7)ThepSecTag2vectorscarrytheZeocinresistancegeneforcost-effectiveselectioninmammaliancells.ZeocinselectioncanalsobeusedinE.coli.ThepSecTag2/HygrovectorshavetheHygromycinBresistancegeneforselectionofstablemammaliancelllines.质粒图谱:上海起福生物科技有限公司联系人:杨建辉400电话:4006551678办公电话:021-50724187传真:021-50724961*8006手机:15921799099企业QQ:4006551678网址:http://www.qfbio.com/地址:上海市浦东新区绣川路561号1102室
标签: