大鼠生长激素释放肽ghrelin试剂盒检测说明书下载
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大鼠生长激素释放肽ghrelin(GHRP-Ghrelin)ELISA试剂盒
大鼠生长激素释放肽ghrelin(GHRP-Ghrelin)酶联免疫分析试剂盒GenericName:Ratgrowthhormonereleasingpeptide-Ghrelin(GHRP-Ghrelin)ELISAKit.实验原理:本试剂盒应用双抗体夹心法测定标本中大鼠生长激素释放肽ghrelin(GHRP-Ghrelin)水平。用纯化的抗-GHRP-Ghrelin抗体包被微孔板,制成固相抗体,往包被单抗的微孔中加入GHRP-Ghrelin,再与HRP标记的羊抗鼠抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成Z终的黄色。颜色的深浅和样品中的GHRP-Ghrelin呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中大鼠生长激素释放肽ghrelin(GHRP-Ghrelin)的含量。PrincipleoftheassayThekitassayRatGHRP-Ghrelinlevelinthesample,usePurifiedRatGHRP-Ghrelintocoatmicrotiterplatewells,makesolid-phaseantibody,thenaddGHRP-Ghrelintowells,CombinedantibodywhichWithHRPlabeledgoatanti-Mousebecomeantibody-antigen-enzyme-antibodycomplex,afterwashingCompletely,AddTMBsubstratesolution,TMBsubstratebecomesbluecolorAtHRPenzyme-catalyzed,reactionisterminatedbytheadditionofasulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm.TheconcentrationofGHRP-GhrelininthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.
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