EGF抗体(F-9): sc-166779

SANTA CRUZ BIOTECHNOLOGY, INC.

EGF抗体(F-9): sc-166779

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com

BACKGROUND

Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid

protein originally found in rodents and humans. It has been shown to be a

potent mitogen for a variety of cell types both in vivo and in vitro. EGF binds

to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation

of the receptor on tyrosine residues. It has been detected in nearly all

body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma.

EGF, TGFα and vaccinia virus growth factor exhibit 30-40% amino acid

homology. Several additional members of the EGF/TGF family have been

described; these include Cripto, Amphiregulin and the heparin-binding EGFlike

growth factor. Amphiregulin and the heparin-binding EGF-like growth

factor both bind to the EGF receptor.

REFERENCES

1. Cohen, S. 1962. Isolation of a mouse submaxillary gland protein accelerating

incisor eruption and eyelid opening in the newborn animal. J. Biol.

Chem. 237: 1555-1562.

2. Gregory, H. 1985. In vivo aspects of urogastrone-epidermal growth factor.

J. Cell Sci. Suppl. 3: 11-17.

3. Stroobant, P., et al. 1985. Purification and characterization of vaccinia virus

growth factor. Cell 42: 383-393.

4. Carpenter, G., et al. 1986. Epidermal growth factor, its receptor, and related

proteins. Exp. Cell Res. 164: 1-10.

5. Derynck, R. 1986. Transforming growth factor α: structure and biological

activities. J. Cell. Biochem. 32: 293-304.

6. Carpenter, G. 1987. Receptors for epidermal growth factor and other polypeptide

mitogens. Annu. Rev. Biochem. 56: 881-914.

7. Starkey, R.H., et al. 1987. Radioimmunoassay of human epidermal growth

factor (urogastrone). J. Clin. Endocrinol. Metab. 45: 1144-1153.

8. Ciardiello, F., et al. 1991. Differential expression of epidermal growth factorrelated

proteins in human colorectal tumors. Proc. Natl. Acad. Sci. USA 88:

7792-7796.

CHROMOSOMAL LOCATION

Genetic locus: EGF (human) mapping to 4q25.

SOURCE

EGF (F-9) is a mouse monoclonal antibody raised against amino acids

971-1023 representing mature EGF of human origin.

PRODUCT

Each vial contains 200 μg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide

and 0.1% gelatin.

STORAGE

Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of

shipment. Non-hazardous. No MSDS required.

APPLICATIONS

EGF (F-9) is recommended for detection of precursor and mature EGF of

human origin by Western Blotting (starting dilution 1:100, dilution range

1:100-1:1000), immunoprecipitation [1-2 μg per 100-500 μg of total protein

(1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution

range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution

range 1:30-1:3000).

Suitable for use as control antibody for EGF siRNA (h): sc-39416, EGF shRNA

Plasmid (h): sc-39416-SH and EGF shRNA (h) Lentiviral Particles: sc-39416-V.

Molecular Weight of EGF precursor: 160 kDa.

Molecular Weight of mature EGF: 6 kDa.

Positive Controls: human platelet extract: sc-363773.

RECOMMENDED SECONDARY REAGENTS

To ensure optimal results, the following support (secondary) reagents are

recommended: 1) Western Blotting: use goat anti-mouse IgG-HRP: sc-2005

(dilution range: 1:2000-1:32,000) or Cruz Marker? compatible goat antimouse

IgG-HRP: sc-2031 (dilution range: 1:2000-1:5000), Cruz Marker?

Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:

sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecipitation:

use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml).

3) Immunofluorescence: use goat anti-mouse IgG-FITC: sc-2010 (dilution

range: 1:100-1:400) or goat anti-mouse IgG-TR: sc-2781 (dilution range:

1:100-1:400) with UltraCruz? Mounting Medium: sc-24941.

DATA

RESEARCH USE

For research use only, not for use in diagnostic procedures.

PROTOCOLS

See our web site at www.scbt.com or our catalog for detailed protocols and

support products.

EGF (F-9): sc-166779. Western blot analysis of EGF

expression in human platelet extract.

132 K

90 K

precursor