采用SPEX GENO做前处理研磨--水稻研究

• Genomics 89 (2007) 532540 ScienceDirect www.sciencedirect.com www.elsevier.com/locate/ygeno Method An efficient method for producing an indexed, insertional-mutant library in rice 水稻插入突变体库的有效建立方法康奈尔大学 在文中，将多个水稻叶片同时在SPEX Geno 2000高通量组织研磨仪内进行研磨，研磨后的样品直接进行DNA提取，可以一次获得大规模的DNA样本由于采用了高通量研磨仪，可以达到GX建立基因文库的效果 Chengkun He, Moul Dey, Zhihong Lin, Faping Duan, Feng领 Li, Ray Wu Department of Molecular Biology and Genetics, Cornell University, 316 Biotechnology Building, Ithaca, NY 14853, USA Received 9 August 2006; accepted 8 November 2006 Available online 16 January 2007