详细介绍
货号: 26906 |
规格: |
基因符号: Gnas |
描述: Anti-Gαs-GTP Mouse Monoclonal Antibody |
背景介绍: Heterotrimeric G proteins are critical cellular signal transducers. Gαs represents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases. |
免疫原: Recombinant full length protein of active Gαs |
经过测试的应用: IP, IHC, IF |
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
浓度: 1 mg/ml |
宿主: |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Anti-active Gαs antibody recognizes active Gαs of vertebrates |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹: Gαs Activation Assay. Purified Gαs proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti Gαs-GTP monoclonal antibody (Cat. No. 26906). Immunoblot was with an anti- Gαs monoclonal antibody (Cat. No. 26006). |
主要用途
GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。它可以结合GTP、水解GTP,因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性,可将GTP水解为GDP,从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。
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仪企号
武汉费斯德生物科技有限公司
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