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- 【导读】髓过氧化物酶(MPO)检测试剂盒检测范围0.781-50ng/mL灵敏度0.35ng/mL样本类型Serum,plasma,tissuehomogenates,celllysate...
髓过氧化物酶(MPO)检测试剂盒
检测范围0.781-50ng/mL灵敏度0.35ng/mL
样本类型Serum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherbiologicalfluids.
实验时长4.5h实验方法双抗夹心法
髓过氧化物酶(MPO)检测试剂盒ELISAKitforMyeloperoxidase(MPO)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!OrganismspeciesHomosapiens(Human)
ProductNo.SEA601Hu
SampletypeSerum,plasma,tissuehomogenates,celllysates,cellculturesupernatesandotherbiologicalfluids.
Format96-wellstripplate
Assaylength4.5hours
Detectionrange0.781-50ng/mLThestandardcurveconcentrationsusedfortheELISA’swere50ng/mL,25ng/mL,12.5ng/mL,6.25ng/mL,3.125ng/mL,1.563ng/mL,0.781ng/mL
SensitivityTheminimumdetectabledoseofthiskitistypicallylessthan0.35ng/mL.髓过氧化物酶(MPO)检测试剂盒Specificity
ThisassayhashighsensitivityandexcellentspecificityfordetectionofMyeloperoxidase(MPO).
Nosignificantcross-reactivityorinterferencebetweenMyeloperoxidase(MPO)andanalogueswasobserved.
Recovery
MatriceslistedbelowwerespikedwithcertainlevelofrecombinantMyeloperoxidase(MPO)andtherecoveryrateswerecalculatedbycomparingthemeasuredvaluetotheexpectedamountofMyeloperoxidase(MPO)insamples.
髓过氧化物酶(MPO)检测试剂盒MatrixRecoveryrange(%)Average(%)
serum(n=5)95-10298
EDTAplasma(n=5)79-9185
heparinplasma(n=5)81-8984Precision
Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelMyeloperoxidase(MPO)weretested20timesononeplate,respectively.
Inter-assayPrecision(Precisionbetweenassays):3sampleswithlow,middleandhighlevelMyeloperoxidase(MPO)weretestedon3differentplates,8replicatesineachplate.
CV(%)=SD/meanX100
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Linearity
ThelinearityofthekitwasassayedbytestingsamplesspikedwithappropriateconcentrationofMyeloperoxidase(MPO)andtheirserialdilutions.Theresultsweredemonstratedbythepercentageofcalculatedconcentrationtotheexpected.
髓过氧化物酶(MPO)检测试剂盒Sample1:21:41:81:16
serum(n=5)98-105%87-97%87-104%88-105%
EDTAplasma(n=5)90-98%94-101%85-104%91-98%
heparinplasma(n=5)98-105%85-97%93-101%91-99%Stability
Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.
Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperature首ldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.
ReagentsandmaterialsprovidedReagentsQuantityReagentsQuantity
Pre-coated,readytouse96-wellstripplate1Platesealerfor96wells4
Standard2StandardDiluent1×20mL
DetectionReagentA1×120μLAssayDiluentA1×12mL
DetectionReagentB1×120μLAssayDiluentB1×12mL
TMBSubstrate1×9mLStopSolution1×6mL
WashBuffer(30×concentrate)1×20mLInstructionmanual1Assayproceduresummary
1.Prepareallreagents,samplesandstandards;
2.Add100μLstandardorsampletoeachwell.Incubate2hoursat37oC;
3.Aspirateandadd100μLpreparedDetectionReagentA.Incubate1hourat37oC;
4.Aspirateandwash3times;
5.Add100μLpreparedDetectionReagentB.Incubate30minutesat37oC;
6.Aspirateandwash5times;
7.Add90μLSubstrateSolution.Incubate15-25minutesat37oC;
8.Add50μLStopSolution.Readat450nmimmediately.
髓过氧化物酶(MPO)检测试剂盒Testprinciple
ThetestprincipleappliedinthiskitisSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoMyeloperoxidase(MPO).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoMyeloperoxidase(MPO).Next,AvidinconjugatedtoHorseradishPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainMyeloperoxidase(MPO),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofMyeloperoxidase(MPO)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.仪器网-专业分析仪器服务平台,实验室仪器设备交易网,仪器行业专业网络宣传媒体。
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- 2004-09-12 09:23:07
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