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Homo sapiens, human

品牌：北京北纳
货号：
CAS编号：
产地：朝阳区
供应商报价：面议

北京北纳创联生物技术研究院更新时间：2022-07-22 10:46:09

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入驻年限第4年

营业执照已审核

同类产品科研细胞(4358件)

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详细介绍

资源编号：BNCC338357

英文名： HTR-8/SVneo

类型： adherent

形态： epithelial-like

分离基物： 6-12 weeks gestation,placenta,trophoblast

安全等级： 2

应用领域： These transfected trophoblasts may be used for the study of trophoblast biology and placental function. They may improve our understanding of diseases related to tumor progression, abnormal placentation hypoinvasiveness in preeclampsia, and hyperinvasiveness in trophoblastic neoplasms.

培养方法

培养基	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
传代方法	Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% (w/v) Trypsin - 0.53 mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times a week
生长条件	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
存储条件	Freeze Medium: Fetal Bovine Serum, 92%; DMSO, 8% Storage Temperature: liquid nitrogen vapor phase

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