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Homo sapiens, human

品牌：北京北纳
货号：
CAS编号：
产地：朝阳区
供应商报价：面议

北京北纳创联生物技术研究院更新时间：2022-07-22 10:46:09

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同类产品科研细胞(4358件)

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详细介绍

资源编号：BNCC342597

英文名： HMC3

类型： adherent

分离基物： brain

培养方法

培养基

The base medium for this cell line is EMEM (ATCC® 30-2003™). To make the complete medium add 56 mL FBS (ATCC® 30-2020™) to a 500 mL bottle of the base medium.

生长条件

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. Cultures can be established between 1 x 104 and 4 x 104 viable cells/cm2. Do not exceed 7 x 104 cells/cm2. 6. Incubate cultures at 37°C.

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