-
-
-
WRL 68
- 品牌：北纳生物
- 型号： BNCC341356
- 产地：北京 朝阳区
- 供应商报价：面议
-
北京北纳创联生物技术研究院
更新时间：2022-07-22 10:46:09 -
销售范围售全国
入驻年限第5年
营业执照已审核
- 同类产品科研细胞(4358件)
立即扫码咨询
联系方式：400-822-6768
联系我们时请说明在仪器网（www.yiqi.com）上看到的!
-
为您推荐
详细介绍
-
资源编号：BNCC341356
英文名： Homo sapiens (human)
类型： adherent
分离基物： Organ: liver
培养方法
传代方法 Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Twice per week 生长条件 The base medium for this cell line is formulated RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C 存储条件 Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
沪公网安备 31011502008050号