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  LZX7899 2019-04-19 16:46:58

  <p><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">1. 培养箱消毒：先用纯水擦洗，再用95的酒精擦洗，再照紫外就可以拉！注意周围环境的清洁就不会那么容易污染的拉！</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">2. 我的经验是在补充水时Z好把水加热一下，达到培养箱的温度。</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">3. 这么早就养细胞了，我们这里一般是用70％的乙醇擦洗，然后再用0.1％的新洁尔灭擦洗，不放心的话再用高锰酸钾加甲醛1：1比例熏一遍。注意要是熏的话千万注意混合之后马上离开，然后密闭细胞培养室，味道很刺激的。Z好在养细胞前消毒，否则，确实没地方放细胞。</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">4. 我们的方法比较简单，先用75%酒精擦拭内壁，通风10分钟，紫外线照射30分钟即可。</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">5. 我们的通常处理是先用75%的酒精擦拭内壁，然后用甲醛+高锰酸钾熏蒸一个晚上，然后通风一整天（同时打开紫外线照射）。</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">6. 我们是把培养箱里的板层拿出来高压一下，箱壁用75％酒精擦一遍，换用诗乐氏擦一遍，再紫外照一夜，效果很好，其他的请高手指教。</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">7. 熏蒸后吹一天是不够的，Z起码要一个星期才可以让甲醛散尽，要不细胞长不好的</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">8. 有的培养箱能加热内壁到90度，也有的带有紫外线功能。常规应该每周用酒精擦洗内部一次。具体的操作请参考培养箱的手册，或着咨询培养箱技术支持。使用消毒剂应小心，培养箱内有检测温度、湿度和二氧化碳浓度的传感器，腐蚀性消毒剂或有机溶剂有可能损坏某些器件，请慎用。另外，挥发性的甲醛有毒，易燃、易爆。如果在培养箱内残留甲醛的话，细胞会死光光哦（我实验室的真实故事）。总之，先看培养箱手册，再联系技术支持，方可万无一失啊。</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">9. 常规Z好什么也不放！硫酸铜的作用是YZ细菌生长（但多数用在处理污染的孔板）。因为现在的孵箱都有YJ功能。</span><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><br style="content: &quot;&quot;; display: block; width: 705px; height: 0px; margin: 15px 0px; color: rgb(51, 51, 51); font-family: &quot;PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="position:relative;line-height:2em;font-size:16px;font-family:Microsoft YaHei;color: rgb(51, 51, 51);PingFang SC&quot;, &quot;Lantinghei SC&quot;, &quot;Microsoft YaHei&quot;, arial, 宋体, sans-serif, tahoma; background-color: rgb(255, 255, 255);" data-x-flag="16_46">10. 我们实验室的做法:把平皿高压灭菌后,在无菌操作台内加入适量的灭菌双蒸水或者生理盐水.再加少量新洁尔灭,浓度没关系.细胞养的都可以.</span></p>

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