C反应蛋白（CRP）检测试剂盒

C反应蛋白(CRP)检测试剂盒

适用生物Chicken(Gallus，鸡)
C反应蛋白(CRP)检测试剂盒检测范围0.625-40ng/mL灵敏度0.273ng/mL
样本类型Serum,plasmaandotherbiologicalfluids.
实验时长4.5h实验方法双抗夹心法C反应蛋白(CRP)检测试剂盒规格96T
ELISAKitforCReactiveProtein(CRP)
FORINVITROANDRESEARCHUSEONLY,NOTFORUSEINCLINICALDIAGNOSTICPROCEDURES!

OrganismspeciesChicken(Gallus)
C反应蛋白(CRP)检测试剂盒SampletypeSerum,plasmaandotherbiologicalfluids.
Format96-wellstripplate
Assaylength4.5hours
Detectionrange0.625-40ng/mLThestandardcurveconcentrationsusedfortheELISA’swere40ng/mL,20ng/mL,10ng/mL,5ng/mL,2.5ng/mL,1.25ng/mL,0.625ng/mL
SensitivityTheminimumC反应蛋白(CRP)检测试剂盒detectabledoseofthiskitistypicallylessthan0.273ng/mL.

Specificity
ThisassayhashighsensitivityandexcellentspecificityfordetectionofCReactiveProtein(CRP).
Nosignificantcross-reactivityorinterferencebetweenCReactiveProtein(CRP)andanalogueswasobserved.
Recovery
MatriceslistedbelowwerespikedwithcertainlevelofrecombinantCReactiveProtein(CRP)andtherecoveryrateswerecalculatedbycomparingthemeasuredvaluetotheexpectedamountofCReactiveProtein(CRP)insamples.

MatrixRecoveryrange(%)Average(%)
serum(n=5)94-10297
EDTAplasma(n=5)95-10299
heparinplasma(n=5)81-10184

Precision
Intra-assayPrecision(Precisionwithinanassay):3sampleswithlow,middleandhighlevelCReactiveProtein(CRP)weretested20timesononeplate,respectively.
Inter-assayPrecision(Precisionbetweenassays):3C反应蛋白(CRP)检测试剂盒sampleswithlow,middleandhighlevelCReactiveProtein(CRP)weretestedon3differentplates,8replicatesineachplate.
CV(%)=SD/meanX100
Intra-Assay:CV<10%
Inter-Assay:CV<12%
Linearity
ThelinearityofthekitwasassayedC反应蛋白(CRP)检测试剂盒bytestingsamplesspikedwithappropriateconcentrationofCReactiveProtein(CRP)andtheirserialdilutions.Theresultsweredemonstratedbythepercentageofcalculatedconcentrationtotheexpected.

Sample1:21:41:81:16
serum(n=5)94-102%80-105%83-93%83-104%
EDTAplasma(n=5)97-105%79-98%96-105%87-97%
heparinplasma(n=5)91-105%89-98%89-103%97-105%

Stability
ThestabilityofkitisdeterminedC反应蛋白(CRP)检测试剂盒bythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.
Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperature首ldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.
Reagentsandmaterialsprovided

ReagentsQuantityReagentsQuantity
Pre-coated,readytouse96-wellstripplate1Platesealerfor96wells4
Standard2StandardDiluent1×20mL
DetectionReagentA1×120μLAssayDiluentA1×12mL
DetectionReagentB1×120μLAssayDiluentB1×12mL
TMBSubstrate1×9mLStopSolution1×6mL
WashBuffer(30×concentrate)1×20mLInstructionmanual1

Assayproceduresummary
1.Prepareallreagents,samplesandstandards;
2.Add100μLstandardorsampletoeachwell.Incubate2hoursat37oC;
3.Aspirateandadd100μLpreparedDetectionReagentA.Incubate1hourat37oC;
4.Aspirateandwash3times;
5.Add100μLpreparedDetectionReagentB.Incubate30minutesat37oC;
6.Aspirateandwash5times;
7.Add90μLSubstrateSolution.Incubate15-25minutesat37oC;
8.Add50μLStopSolution.Readat450nmimmediately.
Testprinciple
ThetestprincipleappliedinthiskitisSandwichC反应蛋白(CRP)检测试剂盒enzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoCReactiveProtein(CRP).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoCReactiveProtein(CRP).Next,AvidinconjugatedtoHorseradishPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainCReactiveProtein(CRP),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitaC反应蛋白(CRP)检测试剂盒changeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofCReactiveProtein(CRP)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.

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