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RecombinantHumanF3/Tissuefactor/CD142CatalogNumber:13133-H02HGeneralInformationGeneNameSynonym:CD142,FLJ17960,TF,TFAProteinConstruction:ADNAsequenceencodingthehumanF3（P13726）extracellulardomain（Met1-Glu251）wasfusedwiththeFcregionofhumanIgG1attheC-terminus.Source:HumanExpressionHost:HumanCellQCTestingPurity:>92%asdeterminedbySDS-PAGE.Endotoxin:1.0EUperμgproteinasdeterminedbytheLALmethodStability:Samplesarestableforuptotwelvemonthsfromdateofreceiptat-70℃PredictedNterminal:Ser33MolecularMass:TherecombinanthumanF3/Fcisadisulfide-linkedhomodimericprotein.Thereducedmonomerconsistsof460aminoacidsandhasapredictedmolecularmassof51.8kDa.Itmigratesasanapproximately66kDabandinSDS-PAGEunderreducingconditionsduetoglycosylation.Formulation:LyophilizedfromsterilePBS,pH7.4Normally5%-8%trehaloseandmannitolareaddedasprotectantsbeforelyophilization.SpecificconcentrationsareincludedinthehardcopyofCOA.Pleasecontactusforanyconcernsorspecialrequirements.SDS-PAGE:UsageGuideStorage:Storeitundersterileconditionsat-70℃uponreceiving.Recommendtoaliquottheproteinintosmallerquantitiesforoptimalstorage.Avoidrepeatedfreeze-thawcycles.Reconstitution:Detailedreconstitutioninstructionsaresentalongwiththeproducts.ProteinDescriptionTissuefactor,alsoknownascoagulationfactorIII,F3,andCD142,isasingle-passtypeImembraneproteinwhichbelongstothetissuefactorfamily.Tissuefactorisoneoftheproteinsthatparticipateinhemostaticandinflammatoryprocesses.Activatedmonocytespresentintheliverincreaseexpressionoftissuefactor,andwhileaccumulatingintheorgantheycanintensifyinflammation.Tissuefactoristheproteinthatactivatesthebloodclottingsystembybindingto,andactivating,theplasmaserineprotease,factorVIIa,followingvascularinjury.Becauseofitsessentialroleinhemostasis,Tissuefactorplaysaroleinpathologyassociatedwithhemostasis,triggeringthecoagulationsysteminmanythromboticdiseasesandthecoagulopathiesassociatedwithsepsisandotherformsofdisseminatedintravascularcoagulation.Tissuefactorisnotonlythemainphysiologicalinitiatorofnormalbloodcoagulation,butisalsoimportantinthenaturalhistoryofsolidmalignanciesinthatitpotentiatesmetastasisandangiogenesisandmediatesoutside-insignal领.Tissuefactorisexpressedconstitutivelybymanytissueswhicharenotincontactwithbloodandbyothercellsuponinjuryoractivation;thelatterincludeendothelialcells,tissuemacrophages,andperipheralbloodmonocytes.References1.Lwaleed,B.A.etal.,2001,JPathol.193（1）:3-12.2.Morrissey,JH.2004,IntJHematol.79（2）:103-8.3.Panasiuk,A.etal.,2007,BloodCoagulFibrinolysis.18（8）:739-44.4.Kinev,AV.etal.,2008,Lupus.17（10）:192211