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- 【导读】RabbitAnti-Vimentin抗体流式说明书,FITCconjugated RabbitAnti-Vimentin抗体流式说明书,FITCconjugatedCat...
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
CatalogNumber:RSF-0272R
Quantitysize:100ug(2mg/ml,Lyophilized.Buffer=0.01MPBS,pH7.4with10mg/mlBSAand0.1%Sodiumazide.)Background:Vimentinisthemajorsubunitproteinoftheintermediatefilamentsofmesenchymalcells.Itisbelievedtobeinvolvedwiththeintracellulartransportofproteinsbetweenthenucleusandplasmamembrane.Vimentinhasbeenimplicatedtobeinvolvedintherateofsteroidsynthesisviaitsroleasastoragenetworkforsteroidogeniccholesterolcontaininglipiddroplets.VimentinphosphorylationbyaproteinkinasecausesthebreakdownofintermediatefilamentsandactivationofanATPandmyosinlightchain-dependentcontractileevent.Thisresultsincytoskeletalchangesthatfacilitatetheinteractionofthelipiddropletswithinmitochondria,andsubsequenttransportofcholesteroltotheorganellesleadingtoanincreaseinsteroidsynthesis.ImmunohistochemicalstainingforVimentinischaracteristicofsarcomas(ofneural,muscleandfibroblastorigin)comparedwithcarcinomaswhicharegenerallynegative.Melanomas,lymphomasandvasculartumorsmayallstainforVimentin.Vimentinantibodiesarethusofvalueinthedifferentialdiagnosisofundifferentiatedneoplasmsandmalignanttumors.Theyaregenerallyusedwithapanelofotherantibodiesincludingthoserecognizingcytokeratins,lymphoidmarkers,S100,desminandneurofilaments.
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
Specificity:•anti-Vimentinisarabbitmonoclonalantibody,FITCconjugated
•specificforVimentinofGoat,mouseandrat
•ProteinGaffinitychromatographypurification,purity:>95%
•Isotype:IgG
•molwt:95kDaApplication:•FCM: 1:20-100
•Immunohistochemistry:1:20-1:200
•Immunocytochemistry:1:20-1:200
•Excitationspectrum:490-495nm
•Emissionspectrum:520-530nm
•Optimalworkingdilutionsmustbedeterminedbytheenduser.
•protectfromlight.RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
Storage:Storeat–20ºCforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20ºC.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibody,theantibodyisstableforatleasttwoweeksat2-4ºC.
ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.
RabbitAnti-Vimentin抗体流式说明书,FITCconjugated
- 2004-07-13 09:31:27
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